Determination of plasma hypoxanthine: A comparison of high-pressure liquid chromatographic and oxygen consumption methods

✍ Scribed by O.D. Saugstad; W.E. Wung; S.B. Howell; L. Gluck

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 366 KB
• Volume: 122
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90265-2

✦ Synopsis

Plasma and serum hypoxanthine (Hyp) concentrations were measured by both high-pressure liquid chromatography and an oxygen consumption (p0,) technique, and the results were compared. The p0, technique is based on the principle that oxygen is consumed when Hyp and xanthine are metabolized to uric acid by xanthine oxidase. Over the concentration range of O-187 pM the slope of the correlation line was 0.94 with a correlation coefficient of 0.99. Over the concentration range O-40 PM, the slope of the correlation line was reduced to 0.87, consistent with the fact that the pot technique is also influenced by the xanthine concentration. However, even when corrected for the xanthine concentration, which averaged 28% of the Hyp concentration, the p02 technique underestimated the Hyp concentration measured by high-pressure liquid chromatography by 5%. The p0, technique has the advantage that it is rapid and simple, however, it has the disadvantage compared to high-pressure liquid chromatography that it does not distinguish between Hyp and xanthine, and it has a lower precision, particularly at the low Hyp concentrations normally present in human plasma.