Determination of plasma and urinary levels of an analgesic anti-inflammatory α[4-(2-isoindolinyl)phenyl]propionic acid derivative by gas—liquid chromatography

✍ Scribed by G.P. Tosolini; A. Forgione; E. Moro; V. Mandelli

• Book ID: 104145050
• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 635 KB
• Volume: 92
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)97189-9

✦ Synopsis

A method for the gas-liquid chromatographic determination of dl-u-[4-(l-oxo-2-isoindolinyl)phenyl]propionic acid in human plasma and urine is described. The substance is extracted from acidified plasma or urine with added internal standard, purified by partition and submitted to gas-liquid chromatography after conversion to its 2,2,2-trifluoroethyl ester. Data on the recovery reproducibility and sensitivity of the method are given. Plasma levels and urinary excretion over time in a subject treated by the oral route with 100 mg of the product are presented.