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Determination of plasma activities of purine nucleoside phosphorylase by high-performance liquid chromatography: estimates of nonparenchymal cell injury after porcine liver transplantation

โœ Scribed by Th. Minor; B. Osswald; T.W. Kraus; N. July; W. Isselhard; E. Klar

Publisher
Elsevier Science
Year
1995
Weight
306 KB
Volume
670
Category
Article
ISSN
0378-4347

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Determination of Plasma Purine Nucleosid
Determination of Plasma Purine Nucleoside Phosphorylase Activity by High-Performance Liquid Chromatography
โœ T. Yamamoto; Y. Moriwaki; S. Takahashi; Y. Nasako; J. Yamakita; K. Hiroishi; K. ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 381 KB

A high-performance liquid chromatographic method was developed for the determination of plasma purine nucleoside phosphorylase activity. In this method, the reaction mixture consisted of \(15 \mu\) l of plasma and \(285 \mu \mathrm{l}\) of \(50 \mathrm{mM}\) phosphate buffer ( \(\mathrm{pH} 7.4\) )