Determination of phosphodiesterase activity in the presence of phosphomonoesterase using bis-p-Nitrophenyl phosphate
β Scribed by Luben B. Dolapchiev; Rositsa A. Vassileva; Dimiter Dimitrov
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 231 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0301-4851
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## Abstract Under the conditions described the determination of calcium in the presence of an equivalent amount of magnesium, using Calβred as indicator, could tolerate up to 2.0 mg. of iron, 1.0 mg. of manganese, 3 mg. of aluminium and up to 6 mg. of phosphate in 50 ml. of solution.
The kinetics of alkaline phosphatase catalysed hydrolysis of p-nitrophenyl phosphate in aqueous carbonatebicarbonate buffered medium as well as in (buffered water-AOT-decane) microemulsion has been studied. Enhancement of reaction turnover has been found in the latter which depends on the mole ratio