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Determination of phenylalanine in river water by high-performance liquid chromatography

✍ Scribed by P.J. Rennie

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
241 KB
Volume
461
Category
Article
ISSN
1873-3778

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✦ Synopsis

The use of o-phthalaldehyde derivatisation of phenylalanine followed by highperformance liquid chromatography with fluorescence detection as used for amino acid analysis in clinical laboratories was examined to assess its suitability for the monitoring of river pollution by the dairy industry by measuring the phenylalanine levels in river water. The method is described together with its statistical evaluation.

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A method is described for the determination of linear aldehydes (C,-C,) in waters. Aldehydes are extracted into n-pentane after derivatization with 2,4-dinitrophenylhydrazine and quantified by reversed-phase high-performance liquid chromatography with detection at 365 nm. With a 250-ml sample, the l