## Abstract The author elaborated a method for separation and determination of peptides in trichloroacetic acid extracts of fish meat by Amido Black 10B. The peptides extracted as Amido Blackβpeptide complexes in acidic medium (APCa) may be determined indirectly according to quantity of bound dye,
Determination of peptides in fish and fish products. Part II. Application of a turbidimetric method for the determination of polypeptides in trichloroacetic acid fish meat extracts
β Scribed by Kolakowski, E.
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 703 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0027-769X
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β¦ Synopsis
Abstract
The method is based on the fact that polypeptides which are soluble in 4% trichloroβacetic acid (TCA) and certain proteins are precipitated if the TCA concentration is increased. The maximum turbidity produced by TCA at a concentration of 25% is measured at 440 nm. Bacitracin B is used for establishing a calibration curve. The method permits to determine peptides with molecular weights ranging from 1000 to 1500; the amino acid compositions of the latter were determined qualitatively.
π SIMILAR VOLUMES
Die berechneten statistischen Gr6Ben sind in gleicher Dimension und mit gleicher Anzahl an Dezimalstellen anzugeben.
## Abstract The possibilities of the direct determination of the thiobarbituric acid (TBA) value in trichloracetic acid (TCA) extracts instead of distillates were evaluated. Tests carried out on herring (Clupea harengus L.), redfish (Sebastes marinus L.), cod (Gadus morhua L.), plaice (Pleuronectes