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Determination of particle distributions in microfluidic systems under the influence of electric fields

✍ Scribed by Andreas Heeren; Monika Fleischer; Dieter Kern

Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
351 KB
Volume
85
Category
Article
ISSN
0167-9317

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✦ Synopsis

One of the challenges in biological applications of nanotechnology is the manipulation of micro and nanoparticles in microfluidic systems. In one approach, the short-range forces exerted on particles by electric fields, e.g. via dielectrophoresis, can be utilized for this purpose. By a combination of dielectrophoresis and electroosmosis it is possible to act on particles in larger volumes. In this work a new method for examining particle distributions in microfluidic devices is presented together with results of experiments in which the method was used to investigate the combination of dielectrophoresis and electroosmosis.

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## Abstract This report investigates the effects of sample size on the separation and analysis of individual biological particles using microfluidic devices equipped with an orthogonal LIF detector. A detection limit of 17β€…Β±β€…1 molecules of fluorophore is obtained using this orthogonal LIF detector