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Determination of oxidized and reduced nicotinamide adenine dinucleotide in cell monolayers using a single extraction procedure and a spectrophotometric assay

โœ Scribed by Ken Umemura; Hideo Kimura


Book ID
108047782
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
195 KB
Volume
338
Category
Article
ISSN
0003-2697

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Several methods are available for the extraction and quantitation of oxidized and reduced pyridine nucleotides in erythrocytes. Enzymatic methods, however, are complicated by the presence of hemoglobin, which causes oxidation of NADH and NADPH during extraction. Although hemoglobin-mediated oxidatio