Determination of oil oxidation by an aldehyde-requiring mutant of luminous bacteria

✍ Scribed by Berkovich, A ;Cogan, U. ;Ulitzur, S.

• Book ID: 101716049
• Publisher: John Wiley and Sons
• Year: 1989
• Weight: 292 KB
• Volume: 3
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170030306

✦ Synopsis

A simple, rapid bioluminescence test (BT) for the determination of lipid oxidation is described. The test utilizes an aldehyde-requiring dark mutant of Vibrio harveyi (M42) that emits light in the presence of long chain (C&,B) aliphatic aldehydes. The procedure consists of treating the oil or fat with Co2+ ion in ethanolic medium at alkaline pH. This treatment facilitates the decomposition of the hydroperoxides into long-chain aldehydes, part of which is used by the bacteria t o produce light. The test was evaluated with corn, soybean and safflower oils, and shows excellent correlation with the commonly used peroxide value assay.