Determination of Nɛ-homocysteinyl-lysine and γ-glutamylcysteine in plasma by liquid chromatography with UV-detection
✍ Scribed by Głowacki, R.; Borowczyk, K.; Bald, E.
- Book ID
- 125394518
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2014
- Tongue
- English
- Weight
- 323 KB
- Volume
- 69
- Category
- Article
- ISSN
- 1061-9348
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A liquid chromatographic (LC) method to assay homocysteine in plasma at micro-molar levels is described. The procedure consists of protein precipitation with trichloroacetic acid and a centrifugation step. The supernatant is then derivatized with a thiol-specific fluorochromophore and LC assayed wit