Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system

✍ Scribed by X. D. Shao; H. Y. Liu; X. F. Gao; W. Q. Chen; Z. H. Song

• Book ID: 111491072
• Publisher: Versita
• Year: 2007
• Tongue: English
• Weight: 191 KB
• Volume: 61
• Category: Article
• ISSN: 0366-6352
• DOI: 10.2478/s11696-007-0047-y

✦ Synopsis

Abstract

A sensitive chemiluminescence method, based on the enhancive effect of norfloxacin on the reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of norfloxacin. The increment of the chemiluminiscence intensity was proportional to the concentration of norfloxacin, giving a calibration graph linear over the concentration from 0.4 ng mL−1 to 400.0 ng mL−1 (r 2 = 0.9988) with the detection limit of 0.1 ng mL−1 (3 × σ noise). At the flow rate of 2.0 mL min−1, a complete determination of norfloxacin, including sampling and washing, could be accomplished in 30 s with the relative standard deviation lower than 3.0 %. The proposed method was applied successfully to determine norfloxacin in pharmaceuticals, human urine, and serum. Possible mechanism of the reaction was also discussed.