Determination of non-volatile N-nitrosamines in baby bottle rubber nipples and pacifiers by high-performance liquid chromatography—thermal energy analysis
✍ Scribed by Nrishinha P. Sen; Stephen W. Seaman; Santosh C. Kushwaha
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 674 KB
- Volume
- 463
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
A method is described for the determination of non-volatile N-nitrosamines in baby bottle rubber nipples and pacifiers. It consists of extraction of the sample with dichloromethane in the presence of ascorbyl palmitate (an inhibitor of artifactual formation of nitrosamines), clean-up on silica or basic alumina, and final analysis by high-performance liquid chromatography-thermal energy analysis, a technique which is highly specific for N-nitroso compounds. The method worked well for the determination of four rubber-related non-volatile nitrosamines, namely, N-nitrosomethylphenylamine, N-nitrosoethylphenylamine, N-nitrosodicyclohexylamine, and N-nitrosodibenzylamine (recoveries from spiked samples > 80%; detection limit, cu. 5 pg/kg for each). Eighteen out of twenty four samples analyzed were found to contain varying levels (mean, 41 pg/kg; range, 8-146 pg/kg) of N-nitrosodibenzylamine. The identity of the compound was confirmed by gas chromatography-thermal energy analysis as well as by gas chromatography-mass spectrometry analyses.