Determination of Nitrate Ion in Marine Biotopes with High Nitrate Content by Ultraviolet Spectrophotometry

Characterization of derivatization of si

Characterization of derivatization of sialic acid with 2-aminoacridone and determination of sialic acid content in glycoproteins by capillary electrophoresis and high performance liquid chromatography - ion trap mass spectrometry

✍ Fa-Yun Che; Xiao-Xia Shao; Ke-Yi Wang; Qi-Chang Xia 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 187 KB 👁 2 views

A simple and highly sensitive capillary electrophoresis (CE) method for determining the content of N-acetylneuraminic acid (Neu5Ac) in glycoproteins was developed. Neu5Ac was derivatized with 2-aminoacridone (AMAC) by reductive amination, and the AMAC-Neu5Ac adduct could be readily separated from th