Determination of myosin light chain phosphorylation using astronomical image analysis programs on autoradiographs of electrophoretic gels
✍ Scribed by Robert D. Bremel; E.Octy Owens; Gillian M. Olins; Christopher M. Anderson
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 608 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0010-4809
No coin nor oath required. For personal study only.
✦ Synopsis
Computer image analysis programs developed by astronomers for celestial photometry were used for the analysis of autoradiographs of electrophoretic slab gels. Oxytocin-stimulated myosin light chain phosphorylation in mammary myoepithelial cells was studied by incubating cells with [32P]orthophosphate followed by oxytocin addition, polyacrylamide gel electrophoresis of cellular protein, and autoradiography of electrophoretic slab gels. After scanning and digitization of autoradiographs, [3ZP]phosphate incorporation into the myosin light chain was measured by the determination of radiation flux recorded on the X-ray film. Within seconds after oxytocin addition, the myosin light chain was fully phosphorylated. The large library of astronomical computer programs has applications for the quantitative analysis of both one-and two-dimensional electrophoretic gels.