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Determination of myo-inositol phosphates in food samples by flow injection-capillary zone electrophoresis

✍ Scribed by Bartolomé M. Simonet; Angel Ríos; Félix Grases; Miguel Valcárcel

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
92 KB
Volume
24
Category
Article
ISSN
0173-0835

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✦ Synopsis

Abstract

A capillary electrophoresis (CE) method with indirect photometric detection was developed to identify and quantify myo‐inositol phosphates in food samples. A flow‐injection (FI) system including a micro‐column containing anionic exchange resin was used for the solid‐phase extraction of the myo‐inositol phosphates with a view to their preconcentration. The FI system was automatically coupled to CE equipment via a mechanical interface. The overall analysis time was shortened by incorporating an FI system for myo‐inositol hexakisphosphate monitoring. The limit of detection for myo‐inositol phosphates as determined by FI‐CE ranged from 11 to 26 μmol/L and the coefficient of variation from 3.9 to 5.0%. On the other hand, the limit of detection and coefficient of variation for myo‐inositol hexakisphosphate as monitored by the FI system were 75 μmol/L and 2.9%, respectively. The proposed method was successfully applied to a variety of food samples with recoveries ranging from 96.0 to 107.7% and the precision from 3.9 to 7.9%. Based on the results, the content of myo‐inositol hexakisphosphate in nuts was two or three times higher than that in legumes.

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