β Scribed by Margaret M. Krahn; Jerome V. Schnell; Marianne Y. Uyeda; William D. MacLeod Jr.
No coin nor oath required. For personal study only.
Nonradiometric techniques were used to identify and quantitate two aromatic hydrocarbons and several of their metabolites in biological samples. High-performance liquid chromatographic separation combined with ultraviolet fluorescence detection provided a sensitive and selective method of trace analysis. Metabolites of benzo[a]pyrene and 2,6-dimethylnaphthalene were prepared by in vitro incubation of these substrates, singly and together, with liver microsomes of coho salmon (Oncorhynchus kisutch). Individual metabolites were separated by high-performance liquid chromatography and quantitated at fluorescence excitation and emission wavelengths characteristic of the corresponding parent hydrocarbon. Future refinement of these techniques may allow the determination of more complex mixtures of xenobiotics and their metabolites in marine organisms.
Large numbers of xenobiotics which enter the marine environment can be found in seawater, sediments, and organisms (l-3). Many of these foreign substances are resistant to chemical and biochemical changes and persist indefinitely.
Others, such as polynuclear aromatic hydrocarbons (PAHs),~ are readily transformed through ' Author to whom correspondence should be sent.
π SIMILAR VOLUMES
A sensitive and selective high performance liquid chromatographic method for the simultaneous determination of nitrated polycyclic aromatic hydrocarbons and their reduced compounds has been developed. As the model compounds for the proposed method, pyrene, aminopyrene, nitrosopyrene, and several nit
Atrasentan is an endothelin antagonist selective for the ET A receptor in development at Abbott Laboratories for the treatment of cardiovascular disease and cell proliferation disorders. A simple and sensitive chromatographic method for the determination of atrasentan in human plasma has been develo