Determination of microcystins in cyanobacterial samples using microliquid chromatography

✍ Scribed by Corinne Rivasseau; Marie-Claire Hennion; Pat Sandra

• Book ID: 102660764
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 813 KB
• Volume: 8
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1996)8:8<541::aid-mcs4>3.0.co;2-1

✦ Synopsis

A method using microliquid chromatography followed by ultraviolet detection (micro LC/UV) has been developed for the analysis of potent cyanobacterial toxins known as microcystins. The technique, which uses a 0.32-mm i.d. column, enables the detection of 10 pg of microcystins-YR and -LR and 50 pg of microcystin-RR. These values correspond to direct injection of 200 nL of 50and 250-pg/L solutions, respectively. The coupling of microchromatography with ionspray and mass spectrometry has also been preliminarily examined. A detection limit of 1 mg/L can be reached for microcystin-LR. The production of toxins by three strains of Cyanobacteria, namely Microcystis aeruginosa PCC7806, M. aeruginosa PCC7813, and Oscillatoria agardhii PCC7805 has been evaluated by micro LC/UV. Microcystin-LR was present in the cellular materials at concentrations of 42, 17, and 6 pg/g dried weight, respectively.