Determination of membrane permeability to water from osmotically induced light-scattering changes of membrane vesicles: Analysis of the method
โ Scribed by E. M. Sorokin
- Book ID
- 110198673
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2008
- Tongue
- English
- Weight
- 243 KB
- Volume
- 55
- Category
- Article
- ISSN
- 1021-4437
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๐ SIMILAR VOLUMES
## Abstract The diffusive permeability to water molecules, P~d~, of lipid vesicles with entrapped paramagnetic solute ions can be determined rapidly from analysis of the magnetic field dependence (nuclear magnetic relaxation dispersion, or NMRD profile) of T~1~ of exterior solvent water protons. Su
A convenient, large-scale method for the isolation of membrane vesicles permeable to specific inorganic ions has been developed. The general principle of this method involves the exchange of Na+ within the vesicles for external Cs+. Vesicles in which this exchange rapidly occurs can be separated on