𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of Lysozyme Using Microcantilever Sensor Based on Atomic Force Microscopy

✍ Scribed by Cheng-Yin WANG; De-Yan WANG; Guo-Xiu WANG; Xiao-Ya HU

Publisher
Elsevier Science
Year
2010
Tongue
Chinese
Weight
344 KB
Volume
38
Category
Article
ISSN
1872-2040

No coin nor oath required. For personal study only.

✦ Synopsis

A microcantilever sensor was developed to detect lysozyme in a pharmaceutical formulation based on the principle of atomic force microscopy. By measuring the cantilever bending and deflection using the optical reflection technique, the lysozyme adsorption on a dodecanethiol-modified cantilever surface was detected. Under optimal conditions, a good linear relationship between the deflection of the microcantilever and the logarithm of the lysozyme concentration in the range from 10 ng L -1 to 0.1 mg L -1 , with a correlation coefficient of 0.998, was established. The limit of detection was 5.0 ng L -1 . The lysozyme in the pharmaceutical formulation sample was determined with the desirable results by using the present method.

πŸ“œ SIMILAR VOLUMES

Determination of Fractured Steel Surface
Determination of Fractured Steel Surface Roughness by Atomic Force Microscopy Using Fractal-based Approaches
✍ Gollion, Ph.; Grenet, G. πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 382 KB πŸ‘ 2 views

Roughness images of fractured steel surfaces were recorded at micrometric level (100-10 000 nm) using an atomic force microscope. We have observed a power law variation of the profile root-mean-square YS. the analysis length. If there is no overall tilt of the images, this variation is indicative of

Characterization of crystals based on 1,
Characterization of crystals based on 1,3,4-oxadiazoles by atomic force microscopy
✍ B. Schulz; B. Stiller; Th. Zetzsche; G. Knochenhauer; L. Brehmer πŸ“‚ Article πŸ“… 1997 πŸ› Elsevier Science 🌐 English βš– 310 KB

The surface structures of crystals based on aromatic oxadiazoles were investigated by MM. The crystal structure for 2,5-di( p-tolyl)-1,3,4-oxadiazole (DTO) differs from that of 2,5-di(4-methoxycarbonyl-phenyl)-1,3,4-oxadiazole (DMPO). In DMPO all molecules show parallel orientation to the surface in