Determination of lipoic acid in chick livers and chicken eggs during incubation
✍ Scribed by Jason C.H. Shih; Scott C. Steinsberger
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 315 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Gas chromatography
of the methyl esters of lipoic ( I ,2-dithiolane-3-pentanoic), bisnor-lipoic ( I .2-dithiolane-3-propanoic), terranor-lipoic (I ,2-dithiolane-3carboxylic) acids. and their reduced (dithiol) forms was carried out on a Poly A-103 column. This new chromatographic method is sensitive and adaptable to determine lipoic acid content in animal tissues and other biological materials.
Livers from l-week-old chicks and eggs at different incubation times were pooled, lyophili7ed. delipidated, hydrolyzed, and extracted. The extracts, after methylation, were chromatographed and quantitated for lipoate. Overall recovery using ['4C]lipoic acid was found to be 347r. The corrected level of lipoic acid in the chick liver was in the range 5-10 @g/g. The level in the egg increased from l-2 @g/egg at 0 day to 22-24 &g/egg at I7 days of incubation.