Determination of leu-enkephalin degradation by a soluble enzyme preparation from calf-brain striatum using reversed-phase high-performance liquid chromatography

✍ Scribed by Jan G.C. Van Amsterdam; Karel J.H. Van Buuren; Jaap Dik; Herman J. Wynne

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 712 KB
• Volume: 258
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96408-2

✦ Synopsis

A rapid,procedure for the determination of Leu-enkephalin (Tyr-Gly-Gly-Phe-Leu) and its main metabolic degradation products (Tyr, Tyr-Gly-Gly and Tyr-Gly) by reversed-phase high-performance liquid chromatography was developed. The method has good precision, the coefficient of variation determined in the range 6-20 pmole being 1.5-3 % (n = S), and a very low detection limit of ca. 10 fmole for each metabolite. An unexpectedly high percentage of Tyr-Gly production is observed after enzymatic degradation of Leu-enkephalin by a solubilized enzyme preparation of calf-brain striatum.