Determination of isoelectric point and investigation of immunoreaction in peanut allergenic proteins–rabbit IgG antibody system by whole-column imaged capillary isoelectric focusing
✍ Scribed by Xing-Zheng Wu; Tiemin Huang; Wayne M. Mullett; Jupiter M. Yeung; Janusz Pawliszyn
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 826 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1040-7685
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✦ Synopsis
Abstract
The recently developed whole‐column imaged capillary isoelectric focusing is an advanced technique for the pI determination of proteins. In this study, we initially applied this technique to determine the pI of peanut allergens and its antibody, rabbit IgG. Two main proteins, Ara h I and Ara h II, were found in the peanut allergen solution. Their pI values were determined to be about 4.5–4.7 and 5.1–5.6, respectively. The value of pI for the antibody rabbit IgG was 6.1–6.5. Second, the immunoreaction selectivity of the two main peanut allergenic proteins with antibody rabbit IgG was studied. The CIEF results show that the peanut allergen Ara h II has a larger affinity for binding to the antibody rabbit IgG than Ara h I. © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 322–326, 2001