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Determination of inorganic phosphate with molybdate and Triton X-100 without reduction

โœ Scribed by Aye Kyaw; Khin Maung-U; Thane Toe

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
301 KB
Volume
145
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A new colorimetric procedure is described for inorganic phosphate determination using the color reaction between inorganic phosphate and acidified ammonium molybdate in the presence of Triton X-100. The method is simple and specific, and produces results comparable with those of the widely used method of Fiske-Subbarow [C. H. Fiske and Y. Subbarow (1925) J. Biol. Chem. 66, 375]. The linearity of the standard curve is observed up to an absorbance of 0.410, compared to 0.370 in the Fiske-Subbarow method. Trichloroacetic acid and tungstic acid are found to interfere in the assay. However, the method is not disturbed by nonionic detergents and can therefore be used for the determination of inorganic phosphate contaminated with nonionic detergents.

๐Ÿ“œ SIMILAR VOLUMES

Determination of inorganic phosphate in
Determination of inorganic phosphate in the presence of triton X-100
โœ Yew Phew See; P.S. Fitt ๐Ÿ“‚ Article ๐Ÿ“… 1972 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 304 KB

During our studies of the polynucleotide phosphorylase of rat liver mitochondrial membranes (1,2), we have needed to assay phosphatase, pyrophosphatase, and nucleoside diphosphatase activities in the presence of Triton X-100 used (2) to solubilize the membrane-bound polynucleotide phosphorylase. The