Determination of Inorganic Phosphate by Coupling Thymidine Phosphorylase and Reversed-Phase High-Performance Liquid Chromatography: Application to Tonoplast Pyrophosphatase Activity

Determination of Pineal Melatonin by Pre

Determination of Pineal Melatonin by Precolumn Derivatization Reversed-Phase High-Performance Liquid Chromatography and Its Application to the Study of Circadian Rhythm in Rats and Mice

✍ Kenji Hamase; Tatsunosuke Tomita; Ayako Kiyomizu; Kiyoshi Zaitsu 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 66 KB

Determination of minute amounts of endogenous melatonin in rat and mouse pineal gland was performed using an RP-HPLC system. Melatonin was separated following precolumn derivatization and determined with a fluorescence detector at the emission wavelength of 380 nm with the excitation at 245 nm. The