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Determination of individual porphyrins in rodent urine using high-performance liquid chromatography following clean-up by anion-exchange chromatography

✍ Scribed by Mark E. Hahn; Thomas A. Gasiewicz


Book ID
107996728
Publisher
Elsevier Science
Year
1991
Weight
377 KB
Volume
563
Category
Article
ISSN
0378-4347

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We have developed a simple method for phosphoamino acid analysis of 32Plabeled phosphoproteins using anion-exchange high-performance liquid chromatography (HPLC). Phosphoproteins undergo partial acid hydrolysis and the resulting hydrolysate is injected directly onto a column. The sample is then isoc