Determination of hydrolyzable tannins in the fruit of Terminalia chebula Retz. by high-performance liquid chromatography and capillary electrophoresis

✍ Scribed by Lih-Jeng Juang; Shuenn-Jyi Sheu; Ta-Chen Lin

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 922 KB
• Volume: 27
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200401741

✦ Synopsis

Determination of hydrolyzable tannins in the fruit of Terminalia chebula Retz. by high-performance liquid chromatography and capillary electrophoresis

A RP-HPLC method for determining fourteen components (gallic acid, chebulic acid, 1,6-di-O-galloyl-D-glucose, punicalagin, 3,4,6-tri-O-galloyl-D-glucose, casuarinin, chebulanin, corilagin, neochebulinic acid, terchebulin, ellagic acid, chebulagic acid, chebulinic acid, and 1,2,3,4,6-penta-O-galloyl-D-glucose) in the fruit of Terminalia chebula Retz. is described. The separation was achieved within 80 min using a binary gradient with mobile phases consisting of a pH 2.7 phosphoric acid solution and an 80% CH 3 CN solution. Capillary electrophoretic analyses were also attempted, and it was found that CZE (25 mM Na 2 B 4 O 7 , 5 mM NaH 2 PO 4 , pH 7.0) was an efficient method for the separation of gallotannins, while an MEKC method (25 mM Na 2 B 4 O 7 , 5 mM NaH 2 PO 4 , 20 mM SDS, pH 7.0, and 10% acetonitrile) provided a better separation for most of the tannins examined. The HPLC and CE methods developed were both successfully applied to the assay of tannins in commercial samples of Chebulae Fructus.