Determination of hydrazine metabolites of isoniazid in human urine by gas chromatography

✍ Scribed by John A. Timbrell; James M. Wright; Clare M. Smith

• Book ID: 104145289
• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 586 KB
• Volume: 138
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)98007-5

✦ Synopsis

A method is described for the determination of isoniazid, acetylisoniazid, acetylhydrazine, diacetylhydrazine and hydrazine in urine. Isoniazid, acctylhydrazine and hydrazine are reacted in aqueous solution with p-chlorobenzaldehyde to form hydrazones. Following the addition of appropriate internal standards, these hydrazones are then extracted into an organic solvent and determined by gas chromatography using a nitrogen-sensitive detector. Acetylisoniazid and diacetylhydrazine are determined similarly after hydrolysis to isoniazid and acetylhydrazine, respectively.

* Standatd deviation calculated for lowest two points on standard curve. ** Sensitivity as defined in the text.