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Determination of human leukocytic interferon by a microfluorometric immunological method

✍ Scribed by S. V. Skurkovich; A. Ya. Ol'shanskii; R. S. Samoilova; E. I. Eremkina


Publisher
Springer US
Year
1979
Tongue
English
Weight
313 KB
Volume
87
Category
Article
ISSN
0007-4888

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A simple and sensitive reversed-phase HPLC method with UV detection was developed and validated for the quantitation of 5-fluorouracil (5-FC) in human plasma. After acidification and salting out, 5-FU was extracted into ethyl acetate and back-extracted into a basic buffer. The extract was adjusted t