Determination of hexuronic acids in glycosaminoglycans by automated ion-exchange chromatography

✍ Scribed by Bhandaru Radhakrishnamurthy; Edward R. Dalferes Jr.; Gerald S. Berenson

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 452 KB
• Volume: 75
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90066-x

✦ Synopsis

This report describes a procedure for analyzing glucuronic and iduronic acids using the Technicon automated sugar chromatography system. Glucuronic and iduronic acids of standard samples of glycosaminoglycans have been analyzed after hydrolysis by formic acid. The method has been applied to quantitate uranic acids in chondroitin sulfates and dermatan sulfate mixtures obtained by Dowex 1 Cl-column fractionation of glycosaminoglycans from aortas of different animal species. The results are in good agreement with those obtained by the gas-liquid chromatographic technique.