𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of HCV genotype by direct sequence analysis of quantitative PCR products

✍ Scribed by Franco Gargiulo; Maria Antonia De Francesco; Gabriele Pinsi; Caterina Pollara; Luigina Terlenghi; Francesca Perandin; Nino Manca

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
83 KB
Volume
69
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Hepatitis C virus (HCV) genotyping, combined with quantitative evaluation of HCV RNA, may be beneficial for the management of chronic hepatitis C and in the selection of candidates for interferon treatment. In this study, the COBAS AMPLICOR HCV MONITOR™ test, a commercially available quantitative assay for HCV RNA, was used. Amplification products obtained from HCV‐positive cases were subjected to direct sequencing and genotyping based on seven phylogenetically informative regions within the 5′UTR. Results were compared with those obtained by INNO‐LiPA assay. Typing results yielded by both methods were in complete accordance for type and subtype assignment. Twenty‐nine of 500 specimens (5.8%) were unclassifiable and belonged to samples with a titer of <70.000 IU, as determined by quantitative assay. Despite this limitation, the overall gain in efficiency, the low rate of test failure and a better resolution of mixed genotypes all constitute a considerable advantage of this system over the commercial hybridization technique for routine clinical laboratory use. J. Med. Virol. 69:202–206, 2003. © 2003 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Determination of Lewis FUT3 gene mutatio
Determination of Lewis FUT3 gene mutations by PCR using sequence-specific primers enables efficient genotyping of clinical samples
✍ Ammi Grahn; Anders Elmgren; Lena Åberg; Lola Svensson; Per-Anders Jansson; Peter 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 69 KB

We have developed a polymerase chain reaction method using sequence-specific primers (PCR-SSP) for rapid and correct genotyping of the common Lewis (FUT3) gene mutations 59T> > G, 202T> > C, 314C> > T, 508G> > A, and 1067T> > A. The PCR-SSP method was validated on 20 healthy blood donors and 16 non-

Discrepancy of hepatitis C virus genotyp
Discrepancy of hepatitis C virus genotypes as determined by phylogenetic analysis of partial NS5 and core sequences
✍ Yun, Zhibing; Lara, Claudia; Johansson, Bo; Lorenzana de Rivera, Ivette; Sönnerb 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 475 KB 👁 1 views

The use of phylogenetic analyses of partial NS5 and core regions for hepatitis Cvirus (HCV) genotyping was evaluated by analysing seven Honduran and 24 European HCV strains. Core primers were designed with which HCV genotypes 1, 2, and 3 were readily amplified. The reliability of phylogenetic analys

Quantitative analysis of food products b
Quantitative analysis of food products by pulsed n.m.r. rapid determination of oil and water in flour and feedstuffs
✍ Ronald E. Hester; Douglas E. C. Quine 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 379 KB

## Abstract A small process analyser employing the pulsed n.m.r. technique has been used to determine water in several different flour types and both oil and water contents of a wide range of animal feedstuffs. The procedures used for establishing and eliminating cross‐interferences in these analys

A genotype distribution of human papillo
A genotype distribution of human papillomaviruses detected by polymerase chain reaction and direct sequencing analysis in a large sample of common warts in Japan
✍ Keisuke Hagiwara; Hiroshi Uezato; Hajime Arakaki; Shigeo Nonaka; Kimiko Nonaka; 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 74 KB

## Abstract There have been no large‐scale epidemiological studies of human papillomavirus (HPV) genotype distribution of common warts in Japan. A total of 213 patients with common warts (104 males and 109 females) in Japan were studied to detect HPV genotype distribution by polymerase chain reacti