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Determination of glyoxylyl-peptide concentration using oxime chemistry and RP-HPLC analysis

✍ Scribed by Jean-Michel Garcia; Samia Far; Eric Diesis; Oleg Melnyk

Book ID: 105360617
Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 183 KB
Volume: 12
Category: Article
ISSN: 1075-2617
DOI: 10.1002/psc.791

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✦ Synopsis

Abstract

Glyoxylyl‐peptides are useful peptide derivatives in the context of hydrazone, oxime or thiazolidine ligations. We describe a method for the determination of glyoxylyl‐peptide concentration based on the reaction of the α‐oxo aldehyde group with an excess of O‐benzylhydroxylamine. The amount of O‐benzylhydroxylamine necessary to convert the α‐oxo aldehyde group into the corresponding O‐benzyloxime was determined by RP‐HPLC analysis and corresponded to the quantity of glyoxylyl‐peptide used in the experiment. The method is rapid, sensitive, accurate and allows the automated analysis of several samples. Copyright © 2006 European Peptide Society and John Wiley & Sons, Ltd.

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