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Determination of glutathione and glutathione reductase with a silver sulfide membrane electrode

✍ Scribed by Hassan, Saad S. M.; Rechnitz, G. A.

Book ID
125943270
Publisher
American Chemical Society
Year
1982
Tongue
English
Weight
643 KB
Volume
54
Category
Article
ISSN
0003-2700

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Determination of picomole quantitites of
Determination of picomole quantitites of hydroperoxides by a coupled glutathione peroxidase and glutathione disulfide specific glutathione reductase assay
✍ Kenneth G.D. Allen; Ching-Jang Huang; Catherine L. Morin πŸ“‚ Article πŸ“… 1990 πŸ› Elsevier Science 🌐 English βš– 477 KB

A procedure to quantitate picomole amounts of hydroperoxides based on GSSG formation is described. Hydroperoxides are incubated with GSH and glutathione peroxidase, and the GSSG formed is measured by a GSSG-specific glutathione reductase recycling assay. Prior to analysis the remaining GSH is remove