Determination of Glucose at the Ru(NH3)63+-Based Paste Enzyme Electrode
✍ Scribed by Liang Chen; Waldemar Gorski
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 63 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
This paper describes a paste enzyme electrode based on a mediator-assisted electrical communication between redox active centers of glucose oxidase and graphite particles. The mediator is a solid inorganic redox salt composed of the Ru(NH 3 ) 6 3 and Ru(CN) 6 4À ions. The actual redox mediation is hypothesized to be accomplished by the pastesolubilized Ru(NH 3 ) 6 3 /Ru(NH 3 ) 6 2 couple that shuttles electrons between the enzyme and the graphite powder. The paste enzyme electrode operates at a potential of 0.0 V vs. Ag/AgCl/3M NaCl and displays superior analytical properties in terms of high operational stability, freedom from interferences, fast response time, extended long term stability, and ease of preparation.