Determination of glibenclamide in human serum by HPLC

✍ Scribed by Nieder, M. ;Dilger, C. ;Jaeger, H.

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 288 KB
• Volume: 9
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240090209

✦ Synopsis

Theantidiabetic drug glibenclamide can bereliablyquantitated in human serum with high performance liquid chromatography. The serum is buffered and extracted with toluene. The organic solvent is evaporated, the residue dissolved in the mobile phase and an aliquot sampled automatically and chromatographed. UV-detection at 229 m allows a lower limit of quantitation of 5 ng/ml. Precise handling of exact volumes facilitates external calibration. Statistical data for imprecision and inaccuracy are given and illustrate reliable quantification. Application of the method to experimental and clinical pharmacokinetic studies with specific problems is illustrated.