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Determination of Fura-2 dissociation constants following adjustment of the apparent Ca-EGTA association constant for temperature and ionic strength

โœ Scribed by D.L. Groden; Z. Guan; B.T. Stokes


Book ID
113236531
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
934 KB
Volume
12
Category
Article
ISSN
0143-4160

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In this study, we investigated the validity of the fluorescent probe fura-2 in determinations of intracellular Ca(2+) concentrations ([Ca(2+)](i)), at physiological temperatures, in poikilothermic animals living at low temperatures. The K(d) for fura-2 was found to decrease with increasing temperatu