Determination of free malonaldehyde formed in liver microsomes upon CCl4 oxidation

✍ Scribed by T. Ichinose; M. G. Miller; T. Shibamoto

• Book ID: 102291259
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 238 KB
• Volume: 14
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/jat.2550140611

✦ Synopsis

Free malonaldehyde formed in the microsomes prepared from livers of monkey, rat, rabbit, mouse, cow, pig, dog, sheep and horse upon CCI4 oxidation was derivatized by reaction with Kmethylhydrazine to form 1-methylpyrazole which was subsequently analyzed by capillary gas chromatography. Among the livers from animals tested, the monkey and rat livers produced the most malonaldehyde upon CCI, treatment. Horse liver showed the greatest resistance to CCI, oxidation. The gas chromatography method used in the present study exhibited an accurate and specific measurement of free malonaldehyde that might provide an understanding of the biochemical process of in vitro lipid peroxidation.