A fluorimetric method for the determination of low levels of theophylline based on the inhibition of the enzyme activity of alkaline phosphatase is proposed. The approach used is a flow-injection configuration where bovine alkaline phosphatase is immobilized on controlled pore glass. The enzymatic r
Determination of escin based on its inhibitory action on lactose crystallization
✍ Scribed by F. Grases; L. García-Ferragut; A. Costa-Bauzá; R. Prieto; J.G. March
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 294 KB
- Volume
- 288
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
A sensitive and simple kinetic turbidimetric method for the determination of escin, based on its inhibitory action on the crystallization of lactose, is presented. Supersaturated solutions of lactose were prepared by addition of acetone to a stable aqueous lactose solution. The induction period of the crystallization process was measured turbidimetrically in the absence and presence of escin. The method was applied to the determination of escin in pharmaceutical products.
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