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Determination of enterokinase activity by measuring the disappearance of trypsinogen

✍ Scribed by Hubert A. Carchon; Els Jansen; Ephrem Eggermont

Book ID
115827437
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
549 KB
Volume
207
Category
Article
ISSN
0009-8981

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✍ Juris J. Liepnieks; Albert Light 📂 Article 📅 1974 🏛 Elsevier Science 🌐 English ⚖ 609 KB

The preparation of /3-trypsin can be simply accomplished from the activation of bovine trypsinogen with partially purified enterokinase in the presence of STI-Sepharose. Enterokinase catalyzes the specific cleavage of lysine 6-isoleucine 7 and the presence of ST1 prevents autolysis of /3-trypsin by