Determination of enalapril and its active metabolite enalaprilat in plasma and urine by gas chromatography/mass spectrometry

✍ Scribed by Hiroaki Shioya; Masako Shimojo; Yukinori Kawahara

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 256 KB
• Volume: 6
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130060203

✦ Synopsis

The method for the simultaneous determination of angiotensin-converting enzyme (ACE) inhibitor enalapril and its active metabolite enalaprilat in plasma and urine was developed by gas chromatography/mass spectrometry. Enalapril and enalaprilat in plasma and urine were extracted and cleaned up by using Sep-Pak C18 and silica cartridges. Derivatization was carried out using diazomethane and trifluoroacetic anhydride. Detection by selected ion monitoring was selected to m/z 288 (enalaprilat) and 302 (enalapril). The detection limit of enalapril and enalaprilat was 200 pg/mL in plasma and 2 ng/mL in urine. This method was applied to the pharmacokinetic analysis of enalapril and enalaprilat in body fluids.