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Determination of Edible Fat Refining by HPLC of Δ 3, 5-Steradienes

✍ Scribed by Schulte, E.

Publisher
John Wiley and Sons
Year
1994
Weight
441 KB
Volume
96
Category
Article
ISSN
0931-5985

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✦ Synopsis

Steradienes are formed during fat refining by dehydration of sterols. For their determination a simple cleanup and a specific HPLC method are described. The excess of other lipids is removed by chromatography on a silica gel column using petroleum ether as an eluant. After concentrating, the eluate is separated on a reversed phase column with acetonitrileher!. butylmethyl ether using UV-detection at 235 nm. The conditions are so specific, that virtually only peaks of steradienes appear in the last part of the chromatograms. Their areas are summed up and the total content is calculated by using cholestadicne as external standard. The last peak representing stigmastadiene can be calculated separately as well.

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