Determination of D,L-serine in midbrain of Parkinson's disease mouse by capillary electrophoresis with in-column light-emitting diode induced fluorescence detection

✍ Scribed by Shuting Li; Qinglei Yu; Xin Lu; Shulin Zhao

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 415 KB
• Volume: 32
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200800459

✦ Synopsis

Abstract

A capillary electrophoresis method with in‐column light‐emitting diode induced fluorescence detection is described for simultaneous determination of D,L‐serine in the midbrain of a Parkinson's disease mouse. D,L‐Serine was derivatized with fluorescein isothiocyanate, and chiral separation and determination of D,L‐serine derivatives were performed on a laboratory‐built capillary electrophoresis system with in‐column light‐emitting diode induced fluorescence detector using γ‐cyclodextrin as chiral selector. Using this method, the levels of D‐ and L‐serine in the midbrains of Parkinson's disease mice were determined. When compared to controls, the levels of D‐ and L‐serine showed significant differences. The result suggested that the biosynthesis and the transportation of endogenous D,L‐serine may participate in Parkinson's disease pathogenesis.