✍ Scribed by Göran Palmskog; Eric Hultman
No coin nor oath required. For personal study only.
The drug (d,Z)-6-chloro-a-methylcarbazole-2-acetic acid (Ro 20-5720) is used as an antiinflammatory agent. For determination of the substance in the normal dose range, an analytical method with high specificity and a sensitivity in the nanomole range is necessary. Previously described methods for the analysis of the drug lack both specificity and sensitivity'.
The high-performance liquid chromatographic (HPLC) method described here was based on reversed-phase ion-pair partition. This technique gives separation systems_ of high efficiency. The retention is determined by the properties of the mobile phase2A. By the use of fluorimetric detection, high sensitivity can be achieved in the analysis of fluorescent compounds.
The fluorescence of carbazole compounds was described by Bender et aL5.
EXPERIMENTAL
An acetate buffer solution (0.1 M, pH 5.0) was prepared. Diethyl ether
📜 SIMILAR VOLUMES
An isocratic high-performance liquid chromatographic method is described for the quantitative analysis of low concentrations of apovincaminic acid (AVA) in blood plasma. AVA, interfering plasma components and primidone (used as the internal standard) were separated on a reversed-phase column of LiCh