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Determination of dissolved CO2 concentration and CO2 production rate of mammalian cell suspension culture based on off-gas measurement

✍ Scribed by Björn Frahm; Heinz-Christian Blank; Peter Cornand; Wolfram Oelßner; Ulrich Guth; Paul Lane; Axel Munack; Klaus Johannsen; Ralf Pörtner

Book ID
119524273
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
255 KB
Volume
99
Category
Article
ISSN
0168-1656

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CO2 concentration in aqueous samples was derived from determination of the CO;! content in the gas phase at equilibrium with the liquid by gas chromatography. This technique was used to measure low dissolved CO, concentrations, down to 10m6 mol/L, and CO, solubility in microbial culture media The me