Determination of dinucleoside 5′, 5‴-P1, P4- tetraphosphates by 31P and 1H NMR spectroscopy
✍ Scribed by Plateau, Pierre; Gueron, Maurice; Blanquet, Sylvain
- Book ID
- 123009541
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 295 KB
- Volume
- 63
- Category
- Article
- ISSN
- 0300-9084
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A simple method for measuring the cellular content of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) in cultured mammalian cells is described. Ap4A was rapidly extracted by dissolving cell monolayers using 0.1 N NaOH. It was separated from the bulk of cellular components in a single step by selecti
The effects of analogs of diadenosine 5',5'''-p1,p4-tetraphosphate (Ap4A) were examined on the ADP-ribosylation reaction of histone H1 catalysed by purified bovine thymus poly(ADP-ribose)transferase. Among the compounds tested, epsilon Ap4A and ApCH2pppA were shown to be the most efficient inhibitor
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