✦ LIBER ✦

Determination of dimethylarginine levels in rats using HILIC-MS/MS: An in vivo microdialysis study

✍ Scribed by Margherita Zotti; Stefania Schiavone; Filomena Tricarico; Marilena Colaianna; Oceania D'Apolito; Giuseppe Paglia; Gaetano Corso; Luigia Trabace

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 417 KB
Volume: 31
Category: Article
ISSN: 1615-9306
DOI: 10.1002/jssc.200800147

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✦ Synopsis

Abstract

Nitric oxide (NO) is one of the most important mediators and neurotransmitters and its levels change under pathological conditions. NO production may be regulated by endogenous nitric oxide synthase (NOS) inhibitors, in particular asymmetric dimethylarginine (ADMA). Most of the interest is focused on ADMA, since this compound is present in plasma and urine and accumulation of ADMA has been described in many disease states but little is known about cerebrospinal fluid (CSF) concentrations of this compound and of its structural isomer symmetric dimethylarginine (SDMA). To determine the levels of methylarginines, we here present a new hydrophilic interaction chromatography (HILIC)‐MS/MS method for the precise determination of these substances in CSF from microdialysis samples of rat prefrontal cortex (PFC). The method requires only minimal sample preparation and features isotope‐labelled internal standards.

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