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Determination of d(−)-β-hydroxybutyric acid in tissue extracts

✍ Scribed by M.N. Berry

Publisher
Elsevier Science
Year
1963
Tongue
English
Weight
429 KB
Volume
6
Category
Article
ISSN
0003-2697

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✦ Synopsis

The enzymic method of Williamson, Mellanby, and Krebs (1) for the determination of D (-) +hydroxybutyrate is not applicable to material containing relatively large quantities of malate because the p-hydroxybutyrate dehydrogenase preparation used could not be completely freed from malate dehydrogenase. A method for the determination of ,8-hydroxybutyrate which can be used in the presence of malate is described in this paper. /3-Hydroxybutyrate is quantitatively converted to acetoacetate in the presence of ,l3-hydroxybutyrate dehydrogenase, pyruvate, lactate dehydrogenase, and NAD, according to the over-all reaction: /3-hydroxybutyrate

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β-hydroxybutyrate dehydrogenase (BHBDH) catalyzes the interconversion of the ketone bodies acetoacetate (Acac) and D-b-hydroxybutyrate (BHB). In virtually all animals, including mammals and fish (Newsholme and Leech, '83) and freshwater molluscs (Meyer et al., '86), BHBDH exists within the mitochond