Determination of cortisol and associated glucocorticoids in serum and urine by an automated liquid chromatographic assay

✍ Scribed by Ghassan J. Samaan; Dominique Porquet; Jean-François Demelier; Daniel Biou

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 499 KB
• Volume: 26
• Category: Article
• ISSN: 0009-9120
• DOI: 10.1016/0009-9120(93)90019-3

✦ Synopsis

We describe a method for the determination of urinary free cortisol and glucocorticoids in plasma, used in the diagnosis of adrenal disorders, based on automated reverse-phase highperformance liquid chromatography (HPLC). The within-day and day-to-day CVs were less than 5.5 and 8.0%, respectively. The calibration curves for cortisol and 11-deoxycortisol were linear up to 2000 nmol/L. Cortisol concentrations as low as 3.5 nmol/L in 1 mL of plasma or urine can be measured. Correlation of HPLC results for 40 plasma specimens with those by radioimmunoassay showed r = 0.965. This method is sensitive and free from the interference habitually encountered in immunoassays, and can thus be proposed for research and as a potential reference method.