Determination of complexing abilities of ligands for metal ions by flow injection analysis and high-performance liquid chromatography : I. Principle of the substitution reaction method

✍ Scribed by Norimasa Yoza; Toshihiko Miyaji; Yukio Hirai; Shigeru Ohashi

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 621 KB
• Volume: 283
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96244-7

✦ Synopsis

A flow injection system, interchangeable as a high-performance liquid chromatographic detector, was designed for the spectrophotometric determination of colourless ligands (L) such as aminopolycarboxylic acids and polyphosphates and for the determination of their complexing abilities. L was allowed to react with a coloured methylthymol blue complex of magnesium or calcium (MR) to form a colourless metal complex (ML). By the spectrophotometric recording of negative peaks at 605 or 610 nm the rapid analysis of 60 samples per hour was possible. The complexing abilities of ligands and the stability constants of ML complexes can be calculated from their peak heights.