Determination of column selectivity toward polycyclic aromatic hydrocarbons

✍ Scribed by Sander, L. C. ;Wise, S. A.

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 427 KB
• Volume: 11
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240110505

✦ Synopsis

An empirical test is describedfortheevaluationof columnselectivity in reversed-phase liquid chromatography. Using a test mixture of three polycyclic aromatic hydrocarbons (PAH), overall column selectivity toward PAH was assessed for over 20 different commercial C18 columns. Retention behavior was correlated to phase type (i.e., monomeric and polymeric surface modification chemistry) for custom synthesized phases. A classification scheme is proposed in which commercial C18 columns are grouped into three classes based on retention behavior: monomeric-like, polymeric-like, and intermediate phase selectivity toward PAH. Correlation of retention behavior of the test mixture with the separation of PAH mixtures and with more general column properties (e.g.. phase thickness) is discussed. ') Certain commercial equipment, instruments, or materials are identified in this report to specify adequately the experimental procedure. Such identification does not imply recommendation or endorsement by the National Bureau of Standards, nor does it imply that the materials or equipment identified are necessarily the best available forthe purpose.