Determination of clomipramine by flow-injection analysis with acidic potassium permanganate–formic acid chemiluminescence detection

✍ Scribed by Zhongling Ji; Xun Yao; Jianguo Li

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 204 KB
• Volume: 26
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1307

✦ Synopsis

ABSTRACT

A sensitive and simple chemiluminescent (CL) method for the determination of clomipramine has been developed by combining the flow‐injection analysis (FIA) technique, which is based on the CL intensity generated from the redox reaction of potassium permanganate (KMnO~4~)–formic acid in sulphuric acid (H~2~SO~4~) medium. Under the optimum conditions, the linear range for the determination of clomipramine was 0.04–4 µg/mL, with a correlation coefficient of 0.9988 (n = 10) and a detection limit of 0.008 µg/mL (3__σ__), and the relative standard deviation (RSD) for 2.0 µg/mL clomipramine (n = 11) is 1.26%. The proposed method has been successfully applied to the determination of the studied clomipramine in pharmaceutical preparations. The possible reaction mechanism is discussed. Copyright © 2011 John Wiley & Sons, Ltd.